Review



pee12 4 vector  (Lonza)


Bioz Verified Symbol Lonza is a verified supplier
Bioz Manufacturer Symbol Lonza manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Lonza pee12 4 vector
    Pee12 4 Vector, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pee12+4+vector/pEE12%2E4/pm41844634-269-8-10
    Average 96 stars, based on 212 article reviews
    pee12 4 vector - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: Reactivating exhausted tumor-infiltrating T cells by a bispecific DC-T cell engager in mice.
    Article Snippet: .. The entire sequence was then cloned into the pEE12.4 vector (Lonza). ..

    Article Title: Sequential intranasal booster triggers class switching from intramuscularly primed IgG to mucosal IgA against SARS-CoV-2
    Article Snippet: For IPRF expression, murine IFN-α4 was fused to the N-terminus of a CD4 helper epitope (PADRE, pan epitope) and RBD, with each component linked by a (G4S)4 linker. .. The IFN-α-pan-RBD sequence was then cloned into the PEE12.4 vector (Lonza) with a human IgG1 Fc, forming the IPRF fusion protein. ..

    Article Title: Engineered anti-PDL1 with IFNα targets both immunoinhibitory and activating signals in the liver to break HBV immune tolerance
    Article Snippet: .. The entire sequence was cloned into the pEE12.4 vector (Lonza). ..

    Clone Assay:

    Article Title: Reactivating exhausted tumor-infiltrating T cells by a bispecific DC-T cell engager in mice.
    Article Snippet: .. The entire sequence was then cloned into the pEE12.4 vector (Lonza). ..

    Article Title: Sequential intranasal booster triggers class switching from intramuscularly primed IgG to mucosal IgA against SARS-CoV-2
    Article Snippet: For IPRF expression, murine IFN-α4 was fused to the N-terminus of a CD4 helper epitope (PADRE, pan epitope) and RBD, with each component linked by a (G4S)4 linker. .. The IFN-α-pan-RBD sequence was then cloned into the PEE12.4 vector (Lonza) with a human IgG1 Fc, forming the IPRF fusion protein. ..

    Article Title: Pre-clinical data supporting immunotherapy for HIV using CMV-HIV-specific CAR T cells with CMV vaccine
    Article Snippet: .. Briefly, the scFv-Fc of N6 was cloned into the Lonza pEE12.4 vector and transiently transfected using the EXPI293 expression system. ..

    Article Title: Engineered anti-PDL1 with IFNα targets both immunoinhibitory and activating signals in the liver to break HBV immune tolerance
    Article Snippet: .. The entire sequence was cloned into the pEE12.4 vector (Lonza). ..

    Plasmid Preparation:

    Article Title: Reactivating exhausted tumor-infiltrating T cells by a bispecific DC-T cell engager in mice.
    Article Snippet: .. The entire sequence was then cloned into the pEE12.4 vector (Lonza). ..

    Article Title: A tumor-specific pro-IL-12 activates preexisting cytotoxic T cells to control established tumors
    Article Snippet: .. For the generation of pro-IL-12, two plasmids, one encoding p35-(G4S) 3 linker or R2-ECD-linker-p35-(G4S)3) linker-hole Fc and the other encoding R1 ECD-linker-p40-(G4S) 3 liner-knob Fc were inserted into pEE12.4 vector (Lonza). ..

    Article Title: Production of heterodimeric proteins
    Article Snippet: For IgG4 antibodies the VH regions were inserted in the pTomG4 vector (containing the genomic sequence of the human IgG4 constant region in the pEE12.4 vector (Lonza Biologics)). .. Alternatively, in follow-up constructs, vectors were used containing the fully codon-optimized coding regions of the heavy chain (IgG1 or IgG4) in the pEE12.4 vector or the human kappa light chain of HuMab 2F8 or HuMab 7D8 in the pEE6.4 vector (Lonza Biologics). .. Additionally, the codon-optimized VH coding region of HuMab-2F8 together with the codon-optimized sequence of the human IgG1m(za) allotype constant region containing the F405L mutation, were cloned in the pcDNA3.3 vector (Invitrogen), yielding expression vector p33G1za-2F8-F405L.

    Article Title: Sequential intranasal booster triggers class switching from intramuscularly primed IgG to mucosal IgA against SARS-CoV-2
    Article Snippet: For IPRF expression, murine IFN-α4 was fused to the N-terminus of a CD4 helper epitope (PADRE, pan epitope) and RBD, with each component linked by a (G4S)4 linker. .. The IFN-α-pan-RBD sequence was then cloned into the PEE12.4 vector (Lonza) with a human IgG1 Fc, forming the IPRF fusion protein. ..

    Article Title: Targeting the Dendritic Cell-Secreted Immunoregulatory Cytokine CCL22 Alleviates Radioresistance
    Article Snippet: Purpose: Radiation-mediated immune suppression limits efficacy and is a barrier in cancer therapy.. Radiation induces negative regulators of tumor immunity including regulatory T cells Ralph R. Weichselbaum, Ralph.Weichselbaum@uchospitals.edu; Hua Laura Liang, hualiang@uchicago,edu; Yang-xin Fu, Yangxinfu@tsinghua.edu.cn., Yang-xin Fu, MD, PhD, A309 Medicine Building, Tsinghua University, Haidian District, Beijing, China 100084, Hua Laura Liang, PhD, Knapp Center for Biomedical Discovery, 900 E 57th Street, Room 6160, Chicago, IL 60637, Ralph R. Weichselbaum, MD, Knapp Center for Biomedical Discovery, 900 E 57th Street, Room 6160, Chicago, IL 60637.. †These authors contributed equally to this work Competing interests: RRW has stock and other ownership interests with Boost Therapeutics, Immvira LLC, Reflexion Pharmaceuticals, Coordination Pharmaceuticals Inc., Magi Therapeutics, Oncosenescence, Aqualung Therapeutics Corporation, Cyntegron and PersonaDX.

    Article Title: Pre-clinical data supporting immunotherapy for HIV using CMV-HIV-specific CAR T cells with CMV vaccine
    Article Snippet: .. Briefly, the scFv-Fc of N6 was cloned into the Lonza pEE12.4 vector and transiently transfected using the EXPI293 expression system. ..

    Article Title: Engineered anti-PDL1 with IFNα targets both immunoinhibitory and activating signals in the liver to break HBV immune tolerance
    Article Snippet: .. The entire sequence was cloned into the pEE12.4 vector (Lonza). ..

    Article Title: Heterodimeric antibody Fc-containing proteins and methods for production thereof
    Article Snippet: For IgG4 antibodies the VH regions were inserted in the pTomG4 vector (containing the genomic sequence of the human IgG4 constant region in the pEE12.4 vector (Lonza Biologics)). .. Alternatively, in follow-up constructs, vectors were used containing the fully codon-optimized coding regions of the heavy chain (IgG1 or IgG4) in the pEE12.4 vector or the human kappa light chain of HuMab 2F8 or HuMab 7D8 in the pEE6.4 vector (Lonza Biologics). .. To introduce mutations in the hinge and CH3 regions of the antibody heavy chains, Quickchange site-directed mutagenesis kit (Stratagene, La Jolla, CA) was used according to the manufacturer's recommendations.

    Construct:

    Article Title: Production of heterodimeric proteins
    Article Snippet: For IgG4 antibodies the VH regions were inserted in the pTomG4 vector (containing the genomic sequence of the human IgG4 constant region in the pEE12.4 vector (Lonza Biologics)). .. Alternatively, in follow-up constructs, vectors were used containing the fully codon-optimized coding regions of the heavy chain (IgG1 or IgG4) in the pEE12.4 vector or the human kappa light chain of HuMab 2F8 or HuMab 7D8 in the pEE6.4 vector (Lonza Biologics). .. Additionally, the codon-optimized VH coding region of HuMab-2F8 together with the codon-optimized sequence of the human IgG1m(za) allotype constant region containing the F405L mutation, were cloned in the pcDNA3.3 vector (Invitrogen), yielding expression vector p33G1za-2F8-F405L.

    Article Title: Heterodimeric antibody Fc-containing proteins and methods for production thereof
    Article Snippet: For IgG4 antibodies the VH regions were inserted in the pTomG4 vector (containing the genomic sequence of the human IgG4 constant region in the pEE12.4 vector (Lonza Biologics)). .. Alternatively, in follow-up constructs, vectors were used containing the fully codon-optimized coding regions of the heavy chain (IgG1 or IgG4) in the pEE12.4 vector or the human kappa light chain of HuMab 2F8 or HuMab 7D8 in the pEE6.4 vector (Lonza Biologics). .. To introduce mutations in the hinge and CH3 regions of the antibody heavy chains, Quickchange site-directed mutagenesis kit (Stratagene, La Jolla, CA) was used according to the manufacturer's recommendations.

    Variant Assay:

    Article Title: Targeting the Dendritic Cell-Secreted Immunoregulatory Cytokine CCL22 Alleviates Radioresistance
    Article Snippet: Purpose: Radiation-mediated immune suppression limits efficacy and is a barrier in cancer therapy.. Radiation induces negative regulators of tumor immunity including regulatory T cells Ralph R. Weichselbaum, Ralph.Weichselbaum@uchospitals.edu; Hua Laura Liang, hualiang@uchicago,edu; Yang-xin Fu, Yangxinfu@tsinghua.edu.cn., Yang-xin Fu, MD, PhD, A309 Medicine Building, Tsinghua University, Haidian District, Beijing, China 100084, Hua Laura Liang, PhD, Knapp Center for Biomedical Discovery, 900 E 57th Street, Room 6160, Chicago, IL 60637, Ralph R. Weichselbaum, MD, Knapp Center for Biomedical Discovery, 900 E 57th Street, Room 6160, Chicago, IL 60637.. †These authors contributed equally to this work Competing interests: RRW has stock and other ownership interests with Boost Therapeutics, Immvira LLC, Reflexion Pharmaceuticals, Coordination Pharmaceuticals Inc., Magi Therapeutics, Oncosenescence, Aqualung Therapeutics Corporation, Cyntegron and PersonaDX.

    Transfection:

    Article Title: Pre-clinical data supporting immunotherapy for HIV using CMV-HIV-specific CAR T cells with CMV vaccine
    Article Snippet: .. Briefly, the scFv-Fc of N6 was cloned into the Lonza pEE12.4 vector and transiently transfected using the EXPI293 expression system. ..



    Similar Products

    96
    Lonza pee12 4 vector
    Pee12 4 Vector, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pee12+4+vector/pEE12%2E4/pm41844634-269-8-10
    Average 96 stars, based on 1 article reviews
    pee12 4 vector - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Lonza vector pee12 4
    Vector Pee12 4, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pee12+4+vector/pEE12%2E4/sec_filing____1175680_slash_000119312517123300_slash_d353531dex104-332-21-30
    Average 96 stars, based on 1 article reviews
    vector pee12 4 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Lonza gs vector pee12 4
    Gs Vector Pee12 4, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pee12+4+vector/pEE12%2E4/us12331113-583-62-65
    Average 96 stars, based on 1 article reviews
    gs vector pee12 4 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Lonza lonza plasmid vectors
    Lonza Plasmid Vectors, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pee12+4+vector/pEE12%2E4/pmc12071099-84-39-39
    Average 96 stars, based on 1 article reviews
    lonza plasmid vectors - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Lonza pee12 4 chinese hamster ovary cho expression vector system
    Pee12 4 Chinese Hamster Ovary Cho Expression Vector System, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pee12+4+vector/pEE12%2E4/us11987630-578-3-11
    Average 96 stars, based on 1 article reviews
    pee12 4 chinese hamster ovary cho expression vector system - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results